PP-051 - Using fibrin-alginate matrix for development of ovarian tissue derived preantral follicles
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چکیده
منابع مشابه
P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture
Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...
متن کاملSurvival Rate of Preantral Follicles Derived From Vitrified Mouse Ovarian Tissue by Cryotop
Purpose: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by cryotop.Materials and Methods: Ovaries of 14-day-old NMRI mice were separated and divided into three groups fowling as: vitrified, toxicity tested and control groups. Ovaries in the experimental groups were immersed in equilibration and vitrification so...
متن کاملEffects of Alginate Concentration and Ovarian Cells on In Vitro Development of Mouse Preantral Follicles: A Factorial Study
Objective In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. MaterialsAndMethods In the first step, preantral follicles (100-130 µm in diameter) were mechanically isolated from the ovaries of 2-week-old NMRI mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% A...
متن کاملMorphological Study of Isolated Ovarian Preantral Follicles Using Fibrin Gel Plus Platelet Lysate after Subcutaneous Transplantation
OBJECTIVE Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL) as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation. MATERI...
متن کاملP-219: GDF9, BMP15 and Their Receptors Expression During In Vitro Culture of Mouse Vitrified Ovarian Tissue Derived Preantral Follicles
Background: In vitro culture (IVC) of isolated preantral follicles from cryopreserved ovarian tissue might be an efficient method for enhancing mature oocytes in patients who are exposed to infertility. Materials and Methods: Ovaries of 13-day old NMRI mice were removed and randomly placed into control,needle immersed (NIV) and solid surface vitrification (SSV) groups. For vitrification, ovarie...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2016
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(16)30186-9